TNP-ATP reverses mechanical allodynia. Thresholds for mechanical allodynia assessed in rats receiving either control IgG or rAQP4 IgG, with or without Intraspinal administration of TNP-ATP (n = 5–6 each group). Data are expressed as means ± SEM and were analyzed by factorial repeated measures ANOVA with two between-subjects factors [group: rAQP4 IgG and control IgG; TNP-ATP: with or without TNP-ATP] and one within-subjects factor [the same rat’s paired right (Rt) and left (Lt) data]. The main effects of Group (P < 0.001) and TNP-ATP (P = 0.003) and the interaction of Group times TNP-ATP (P = 0.003) were statistically significant. For the visualization, the corresponding two-group comparisons were indicated by the asterisks, **P < 0.0.