Table 1 Primer sequences for qPCR
Gene | Species | Accession number | Primer sequence | Amplicon length, base pairs |
|---|---|---|---|---|
18S | Rat | NR_046237.1 | 5′-GTAACCCGTTGAACCCCATT-3′ 3′-CCATCCAATCGGTAGTAGCG-5′ | 151 |
IL-1α | Sheep | NM_001009808.1 | 5′-GTCCATACATGACGGCTGCTA-3′ 3′-GGTGTCTCAGGCATCTCCTTAT-5′ | 184 |
IL-1 β | Sheep | NM_001009465.2 | 5′-CGATGAGCTTCTGTGTGATG-3′ 3′–CTGTGAGAGGAGGTGGAGAG-5′ | 121 |