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Table 3 Top 20 proinflammatory genes significantly increased in the brain of untreated mice after 6 h of exposure to TLR agonists relative to PBS-treated control mice (n = 6)

From: Innate immune responses after stimulation with Toll-like receptor agonists in ex vivo microglial cultures and an in vivo model using mice with reduced microglia

LPS (n = 7) IMQ (n = 7) CpG-ODN 1585 (n = 6)
Gene FC P value Gene FC P value Gene FC P value
Cxcl10 545.7 2.9E-09 Cxcl10 203.4 4.0E-10 Cxcl10 648.2 < 1.0E-10
Ccl20 189.7 3.3E-07 Ccl20 39.4 1.9E-04 Ccl20 383.3 < 1.0E-10
Il1rn 157.3 6.0E-07 Ccl2 33.0 7.0E-05 Il1rn 115.6 4.0E-07
Cxcl1 95.2 4.9E-06 Ccl7 29.1 1.9E-05 Ccl2 103.3 4.5E-07
Ccl2 91.2 1.4E-05 Il1rn 25.1 1.9E-05 Ccl7 68.2 3.6E-07
Ccl5 82.7 7.7E-08 Ccl4 19.7 4.8E-05 Il1b 66.1 4.3E-06
Csf3 79.9 2.2E-08 Ccl3 18.9 1.4E-07 Ccl4 64.4 1.8E-06
Ccl3 64.6 5.5E-08 Ccl12 16.8 1.1E-05 Ccl3 59.3 8.2E-09
Il1b 58.1 4.3E-06 Tnf 9.6 5.5E-05 Tnf 52.1 1.6E-07
Ccl4 55.4 5.8E-06 Ccl11 9.6 6.4E-04 Csf3 47.0 5.8E-08
Ccl7 52.5 6.3E-05 Cxcl11 9.1 2.7E-03 Cxcl1 46.5 2.9E-06
Ccl11 43.0 2.7E-05 Cxcl13 8.5 3.6E-04 Ccl5 41.5 9.0E-10
Cxcl5 27.7 7.9E-04 Ccl5 7.8 2.6E-06 Ccl11 26.1 2.1E-05
Il1a 25.3 1.5E-06 Csf3 7.6 1.0E-03 Ccl12 21.0 4.4E-07
Tnf 23.8 5.8E-04 Cxcl1 6.8 1.7E-02 Cxcl5 20.8 8.1E-05
Cxcl9 17.7 8.2E-05 Il1b 6.7 1.6E-02 Cxcl11 18.9 1.7E-05
Ccl22 16.7 1.6E-05 Cxcl5 6.1 2.8E-02 Cxcr2 16.9 1.5E-04
Ccl12 16.4 1.7E-04 Tnfsf10 5.2 1.8E-06 Cxcl9 10.8 1.6E-08
Ccl9 15.5 7.9E-04 Il1a 3.9 8.3E-04 Il1a 10.1 7.5E-08
Tnfsf11 9.0 1.2E-03 Ccr5 3.6 1.2E-02 Tnfsf10 9.5 4.4E-08
  1. n is the number of independent mouse RNA samples tested. Fold change (FC) relative to PBS-treated mice. P values are calculated using the student t-test on the Delta CT. All changes reached our criteria of statistical significance where the FC is greater than 2-fold with a P value ≤ 5.0E-02