Fig. 2

Analysis of single-cell RNA sequencing data reveal the expression pattern of IL6-receptor encoding genes in the arcuate nucleus of the hypothalamus. A Diffusion-based manifold approximation and projection (dbMAP) embedding of 20,921 single-cell transcriptomes of the arcuate nucleus of the hypothalamus, colored by their annotated cluster of origin. In this embedding, similar cells are mapped tightly, whereas dissimilar cells are mapped apart from each other, rendering clusters of cell types. Cells were annotated by their main cell type as defined in the original study. B (top) Il6ra mRNA expression, key-colored by expression intensity; Il6ra is found mainly in perivascular macrophages/microglia and ependymocytes and also in astrocytes and endothelial cells. B (bottom) Cx3cr1 (a canonical microglial marker) mRNA expression, key-colored by expression intensity. C (top) Il6st mRNA expression, key-colored by expression intensity; Il6st is broadly expressed in the arcuate with some overlapping with the expression pattern of Ntrk2 (bottom), which encodes a neurotrophic receptor. D Violin plot of the mRNA expressions of Il6, Il6ra, Il6st, Cx3cr1, Vim, Ntrk2, and Gfap in the arcuate hypothalamus, per main cell types. Oligodend, oligodendrocyte; NG2/OPC, oligodendrocyte progenitor cells; PVMMicro, perivascular macrophages/microglia; VLMCs, vascular and leptomeningeal cells; ParsTuber, pars tuberalis