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Fig. 1 | Journal of Neuroinflammation

Fig. 1

From: Hippocampal glucose uptake as a surrogate of metabolic change of microglia in Alzheimer’s disease

Fig. 1

Increased hippocampal glucose uptake in Alzheimer’s disease mediated by microglia. A The overall workflow of the study using [18F]fluorodeoxyglucose (FDG) is represented. FDG was injected into 5xFAD mice of different ages and wild-type (WT) mice. Small-animal PET scans were acquired to non-invasively assess glucose uptake in the hippocampus. In addition, hippocampal cells were dissociated to analyze microglial FDG uptake as an ex vivo study. Single cell RNA-seq analyses were also performed by dissociating hippocampal cells. B To evaluate FDG uptake in the hippocampus on FDG PET, spatially normalized PET images and volume of interest for the hippocampus defined on the template MRI were used. C Four-month-old mice showed no significant difference in hippocampal glucose uptake of 5xFAD and WT (p = 0.28 and p = 0.36 for right and left hippocampus, respectively). Eight-month-old 5xFAD mice showed significantly higher FDG uptake in the hippocampus than WT (p = 0.016 and p = 0.032 for right and left hippocampus). Twelve-month-old 5xFAD mice also showed a similar pattern, with higher FDG uptake in the hippocampus than WT mice (p = 0.0091 and p = 0.064 for right and left hippocampus). D After FDG injection, the hippocampus was dissected, and microglia were dissociated. Ex vivo measurement of FDG activity showed that microglial uptake was significantly higher in 5xFAD at 7.5 months of age. Thirteen-month-old 5xFAD mice showed higher microglial FDG uptake than WT mice, although this difference did not reach statistical significance (p = 0.062). E Non-microglial cells of the hippocampus showed no significant difference in FDG uptake between 5xFAD and WT for all age groups

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