Table 1 Primers for qRT-PCR
Gene | Sense (5′-3′) | Anti-sense (5′-3′) |
|---|---|---|
r-IL-1β | TGACCTGTTCTTTGAGGCTGAC | CATCATCCCACGAGTCACAGAG |
r-IL-6 | CAGCGATGATGCACTGTCAGA | GGAGAGCATTGGAAGTTGGGG |
r-TNF-α | GCCACCACGCTCTTCTGTCTA | CGCTTGGTGGTTTGCTACGA |
r-iNOS | CAGATCCCGAAACGCTACACT | TGAGTTGAACAAGGAGGGTGGT |
r-COX-2 | TCAGCCATGCAGCAAATCCTT | TCCAGTCCGGGTACAGTCACA |
r-Actin | CGTTGACATCCGTAAAGACCTC | TAGGAGCCAGGGCAGTAATCT |
m-IL-1β | TTTGAAGTTGACGGACCCCAA | CACAGCTTCTCCACAGCCACA |
m-IL-6 | TTCTTGGGACTGATGCTGGTG | CACAACTCTTTTCTCATTTCCACA |
m-TNF-α | ATGTCTCAGCCTCTTCTCATTC | GCTTGTCACTCGAATTTTGAGA |
m-iNOS | GGGCTGTCACGGAGATCAATG | GCCCGGTACTCATTCTGCATG |
m-COX-2 | GGGCCATGGAGTGGACTTAAA | TGCAGGTTCTCAGGGATGTG |
m-Actin | CTGAGAGGGAAATCGTGCGT | CCACAGGATTCCATACCCAAGA |