Fig. 5

sEHi TPPU alleviates CPSP-induced mechanical allodynia in a dose and time-dependent manner. a The time schedule of the present experiment. After 7-day acclimation, rats were subjected to thalamic hemorrhage and were then treated with vehicle (0.1% DMSO) or a different dose of TPPU (0.01 mM, 0.1 mM, 1 mM) once daily within 5 consecutive days after stroke. Rats in each treatment were analyzed by von Frey tests for PWMT at 1 day before (baseline, BL), and 7, 14, 21, and 28 days post stroke. b TPPU reduces pain in a dose and time-dependent manner. On day 7 after CPSP, rats treated with a moderate (0.1 mM) or high dose of TPPU (1 mM) exhibited increased PWMT in both hind paws compared to those treated with the vehicle or a relatively low dose of TPPU (0.01 mM). However, this analgesic effect did not last long, with PWMT almost returning to baseline within 9 days after drug withdrawal (at day 14 after CPSP). Data are expressed as mean ± SD, n = 10 per group, two-way ANOVA, followed by Bonferroni tests, *P < 0.05, **P < 0.01, ****P < 0.0001