Table 1 Primers used for real-time polymerase chain reaction (PCR) amplification
From: 1α,25-dihydroxyvitamin D3 protects retinal ganglion cells in glaucomatous mice
Genes | Primer murine sequences |
|---|---|
18S | F: 5′-GTTCCGACCATAAACGATGCC-3′; R: 5′-TGGTGGTGCCCTTCCGTCAAT-3′ |
BDNF | F: 5′-GTTCGAGAGGTCTGACGACG-3′; R: 5′-AGTCCGCGTCCTTATGGTTT-3′ |
VEGF-A | F: 5′-GCACATAGGAGAGATGAGCTTCC-3′; R: 5′-CTCCGCTCTGAACAAGGCT-3′ |
PlGF | F: 5′-ATGCTGGTCATGAAGCTGTTCA-3′; R: 5′-GGACTGAATATGTGAGACACCT-3′ |
IL-6 | F: 5′-CCAGAGCTGTGCAGATGAGTA-3′; R: 5′-TGGGTCAGGGGTGGTTATTG-3′ |
IL-1β | F: 5′-ACATCAGCACCTCACAAGCAGAG-3′; R: 5′-TGGGGAAGGCATTAGAAACAGTC-3′ |
IFN-γ | F: 5′-TGCATCTTGGCTTTGCAGCTCTTCCTCATG-3′; R: 5′-TGGACCTGTGGGTTGTTGACCTCAAACTTG-3′ |
CCL-3 | F: 5′-TGAATGCCTGAGAGTCTTGG-3′; R 5′-TTGGCAGCAAACAGCTTATC-3′ |