Fig. 1

Expression analysis of HSF1 protein levels and colocalization with astrocytes following SCI. a Western blot analysis of HSF1 expression following spinal cord contusion at 0 day, 1 day, 4 days, and 7 days, respectively. M indicates the markers. b Quantification data as shown in (a); quantities were normalized to endogenous β-actin. n = 6. Experiments were performed in triplicates. Error bars represent the standard deviation. *P < 0.05, one-way ANOVA with Dunnett’s post hoc test. F (3, 8) = 61.05, P < 0.0001. 4 days vs 0 day, P < 0.0001; 7 days vs 0 day, P = 0.0002. c Immunostaining of HSF1 in the cross sections of rat contused spinal cord showed colocalization with GFAP-positive cells at 0 day, 1 day , 4 days, and 7 days, respectively. The rectangles indicate region magnified. Arrowheads indicate colocalization of HSF1 with astrocytes. Scale bars, 200 μm or 50 μm in magnification