Fig. 5

Effects of HSF1 agonist 17-AAG on the expression of C3 and S100A10 in the astrocytes. a Toxic assay of 0-10 μmol/L 17-AAG for the astrocytes. b–e Western blot analysis of C3 (b, c), S100A10 (b, d) and HSP70 (b, e) in astrocytes following cell incubation at 0–10 μmol/L 17-AAG for 24 h. Quantities were normalized to endogenous β-actin. Experiments were performed in triplicates. Error bars represent the standard deviation, *P < 0.05, one-way ANOVA with Dunnett’s post hoc test. For C3 quantitative analysis, F (4, 10) = 4.763, P = 0.0207. 0.1 vs con, P = 0.0019; 0.5 vs con, P = 0.0526; 2.5 vs con, P = 0.0432; 10 vs con, P = 0.0066. For HSP70 quantitative analysis, F (4, 10) = 10.89, P = 0.0011. 0.1 vs con, P = 0.0051; 0.5 vs con, P = 0.0032; 2.5 vs con, P = 0.0017; 10 vs con, P = 0.0004