Fig. 4

Inhibition of microglial GCase hamper the neuronal Nfe2l2 response. A Schematic representation of the experiments summarized in (B, C) to assess the effect of the selective GCase inhibition in microglial or neuronal cells. BV-2 (B) or SK-ARE-luc2 (C) cells were pre-treated with 200 μM CBE for 48 h before seeding the cocultures. Data are expressed as fold changes (FC) of RLU versus vehicle and presented as mean ±SD of n=4 independent samples measured in triplicate. *p<0.05 by unpaired t-test. D Schematic representation of the experiments reported in E and F aimed at testing the effect of primary microglia extracted from mice treated with 100 mg/kg/die CBE for 3 days. E GCase activity in the primary microglia expressed as % of the activity detected in microglia extracted from the vehicle treated animals. Data are presented as mean ±SD of n=2 independent samples measured in duplicate. ***p<0.001 calculated by unpaired t-test. F Luciferase activity measured in protein extracts derived from ARE-luc2 neurons cocultured with microglia derived from CBE or vehicle treated mice. Data are presented as mean ±SD of n=4 independent samples measured in duplicate. *p<0.05 calculated by unpaired t-test.