Fig. 5

FAAH deletion enhances the expression of TREM2 in 5xFAD mice. A Expression levels of TREM2 (A), obtained from hippocampal extracts after RT-qPCR. Two-way ANOVA followed by Tukey’s test (**p < 0.01, ****p < 0.0001) (n = 6–8 animals in each group). Graphs represent mean ± s.e.m. B Representative images showing TREM2 protein expression in microglia surrounding amyloid plaques in cortex, as revealed by immunofluorescence. Blue channel (left) corresponds to amyloid plaque labelling with methoxy-XO4; red channel (middle) shows anti-TREM2 staining. Merged images are shown in the right panels. C Densitometric quantification of TREM2 fluorescence. N = 3 mice per group. ****p < 0.0001 by Mann–Whitney test. Error bars represent mean ± s.e.m. Scale bars, 20 μm