Fig. 4

Few kinase families regulate the phosphoproteome in GIFN39 mice. A Sequence logos and fold increase of enriched motifs of the upregulated phosphopeptides calculated by motif-X. Kinase family and matched consensus substrate motif: MAPK/CDK (sP and sP…K), CK (s.E) and CaMK (R..s). B Top 10 kinase families predicted based on substrate motif matches and protein–protein interaction. Percentages of the upregulated phosphopeptides associated with a kinase compared with the total number of phosphopeptides associated with a kinase family are shown. C Kinase activity score of kinases calculated from the abundance, fold change (GIFN39 vs WT) and role, extracted from PhosphoSitePlus, of each phosphosite on the kinase. Dashed line: threshold score equivalent to a 1.5-fold reduced activity