Fig. 7

PKM2 facilitates phagocytosis via the β-catenin signaling pathway. A The mRNA expression levels of β-catenin, c-Myc, and Cyclin-D1 detected by RT-PCR analysis at 24 h after plasmid transfection. B The protein expression of β-Catenin, c-Myc, and Cyclin-D1 detected by Western blot analysis 24 h after plasmid transfection. C Western blot quantification of β-catenin, c-Myc, and Cyclin-D1 in the hippocampus of control and MLP/lpr mice, n = 4. D BV2 cells were treated with different concentrations of β-catenin inhibitor (KYA1797K) for 24 h. CCK8 detected the cytotoxicity of KYA1797K to BV2 cells. E Transient transfection of BV2 cells with the negative control plasmid (NC) or PKM2 over-expressed plasmid (PKM2). After transfection, BV2 cells were treated with or without 200 ng/mL β-catenin inhibitor (KYA1797K) for 24 h. Then, a phagocytic function test was performed, and the phagocytosis of BV2 cells was detected by flow cytometry. F Transient transfection of BV2 cells with NC or PKM2 over-expressed plasmid, 24 h later, BV2 cells were treated with or without 200 ng/mL β-catenin inhibitor (KYA1797K) for 24 h. The expression level of LAMP1 was detected by flow cytometry. G BV2 cells were transiently transfected with NC or PKM2 over-expressed plasmid (PKM2); 24 h later, BV2 cells were treated with or without 200 ng/mL KYA1797K for 24 h, and then co-cultured with HT22 cells. Finally, a Western blot was used to analyze the protein level of PSD95. Data are presented as mean scores ± SEM, *P ≤ 0.05, **P ≤ 0.01, ***P ≤ 0.001, ****P ≤ 0.0001. n = 3 per group