Fig. 1

P2ry6 knockout mice are protected against peripheral LPS-induced neuronal loss. WT and P2ry6^−/− mice were injected i.p. with LPS on 4 successive days and 14 days later the substantia nigra was sectioned and stained to quantify neuronal and microglial density. a Representative images of anatomically matched, anti-tyrosine hydroxylase (TH) stained sections of substantia nigra pars compacta (SNpc) in LPS-treated and vehicle-treated WT and P2ry6^−/− mice. Scale bar: 100 µm. b TH-positive dopaminergic neurons per section in SNpc. c Relative densities of NeuN-positive neurons in SNpc. c Relative densities of Iba-positive microglia (or other macrophages) in SNpc. Each data point represents one animal and error bars represent mean ± SEM. Statistical analysis was performed using two-way ANOVA with post hoc Tukey’s multiple comparison test. *p < 0.05, **p < 0.01, ****p < 0.0001