Fig. 3

Volume of ganglionic macrophages enlarges after nerve injury. Iba1-positive cells (red) in the contralateral (contra) and ipsilateral (ipsi) sides of the maxillary nerve region of the trigeminal ganglion on day 7 after infraorbital nerve ligation (a) and the cell areas of Iba1-positive cells (n = 4–6/timepoints) (b). Z-stack images showing Iba1-positive cells (red) around ATF3-positive cells (green) (c), three-dimensional images showing Iba1-positive cells (d), and the cell volume of Iba1-positive cells (n = 10 cells/group from 3 mice) (e) on day 7 after nerve ligation. See list of abbreviations. Scale bars are indicated. Data are represented as mean (S.D.), and differences were detected using two-way ANOVA with Tukey–Kramer test (b) and Student’s t test with Welch’s correction (e)