Fig. 3

Less and shorter regenerating axons in the crush injured sciatic nerve in cKO group. a, b Western blots and quantification showing the levels of GAP43 in the injured nerve at 3 dpi. c GAP43 immunohistochemistry in the longitudinal sections of the injured nerves. Scale bars: 400 µm. d The length of the longest GAP43 positive axons from the crush injured site (labeled with yellow line). e The number of GAP43 positive axons crossing the lines at 2 mm, 4 mm, 6 mm distal to the injury site. Data are expressed as the mean ± SEM (n = 6 per group)