Fig. 3

Ketamine-induced pyroptosis in PC12 cells is NLRP3/Caspase-1 inflammasome-dependent. A Cell activity analysis in PC12 cells. B LDH release test. C Western blotting analysis of NLRP3-3, ASC, cleaved Caspase-1, and cleaved GSDMD (n = 3) D Effects of NLRP3/Caspase-1 Inflammasome inhibitors on ketamine-induced double-positivity for PI and activated caspase-1 flow cytometry analysis. E The cytokines levels of IL-1β and IL-18 in PC12 cells (n = 6). Values are expressed as the mean ± SD. *P < 0.05, **P < 0.01, compared with group C; ^# P < 0.05, ^##P < 0.01, compared with group Ket. Con, control; Ket, ketamine