Fig. 5

MiR-204-5p overexpression within the hippocampal DG region alleviates neuronal inflammation and oxidative stress in CUS rats. A Representative confocal microscopic images showing expressions of 4-HNE in the DG region after AAV-miR-204-5p infusion (N = 6 per group). Scale bar is 50 μm. B Representative images of Mito-Sox staining (red). Nuclei (blue) are stained with DAPI. Neurons (green) are stained with Anti-NeuN. Scale bar is 50 μm. (N = 6 per group) C Representative confocal microscopic images of immunostainings for 8-oHdG in the DG region. Scale bar = 50 μm. (N = 6 per group) D Immunofluorescent staining of Iba1 positive microglial cells within the DG region. Scale bar is 50 μm. (N = 6 per group) E Western bolt assays of protein expression levels of NOX1 and NOX4 within the DG region (N = 6 per group). F Q-PCR showing mRNA levels of pro-inflammatory cytokines, Band intensities were normalized to GAPDH. N = 6 per group, ^****P < 0.0001, CUS + AAV-control vs CUS + AAV-miR-204-5p. Data are presented as means ± SEMs. Student t-tests were employed for comparisons between the two groups. One-way ANOVA followed by Tukey’s post-hoc test was used for multiple comparisons involving > 2 groups