Fig. 6

MiR-204-5p inhibits the occurrence of oxidative stress within the hippocampal DG region via the RGS12/Nrf2-NF-κB pathway. A Representative images of RGS12 (red) and MAP2 (green) staining. Nuclei (blue) are stained with DAPI. Scale bar is 50 μm. (N = 6 per group) B, C Representative western blot images showing relative protein levels of RGS12, Nrf2, NQO1, HO-1 and NF-κB p65 in AAV-miR-204-5p sponge virus infected DGs (N = 6 per group). (D-E) Representative western blot images showing relative protein levels in AAV-miR-204-5p virus infected DGs (N = 6 per group). **P < 0.01 ***P < 0.001, ****P < 0.0001, control + AAV-control vs control + AAV-miR-204-5p sponge; ^&&P < 0.01, ^&&&P < 0.001, ^&&&&P < 0.0001, CUS + AAV-control vs CUS + AAV-miR-204-5p. Data are presented as means ± SEMs. Student t-tests were employed for comparisons between the two groups. Two-way ANOVA was used for Fig. 6A. One-way ANOVA followed by Tukey’s post-hoc test was used for C and E