Fig. 2
From: Altered substrate metabolism in neurodegenerative disease: new insights from metabolic imaging
FLIM imaging and analysis. Mouse hypothalamus (A) is excited (B) with 2 photon laser to collect NADH and FAD fluorescence signal on multiple smaller fields of view (C) and measure their lifetimes. Phasor plots for NADH (D) and FAD (E) and corresponding lifetime maps distributions (F and G, respectively) are created