Fig. 2

Effect of macrophage polarization on the OT signalling system. A Schematic diagram illustrating the procedures used to obtain conditioned medium. B–E The levels of OT and OTR proteins in cultured enteric neurons treated with or without conditioned medium were detected by western blot. M1 supernatant inhibited the protein expression of OT and OTR; by contrast, M2 supernatant promoted the protein expression of OT and OTR. F Representative photographs of double immunofluorescent staining of tubulin (green) and oxytocin (OT) (red) within randomly captured images were obtained in cultured enteric neurons. Scale bar = 20 μm. G, H The mRNA levels of OT and OTR in cultured enteric neurons treated with or without conditioned medium for 24 h were detected by qRT-PCR. M1 supernatant inhibited the mRNA levels of OT and OTR; by contrast, M2 supernatant promoted the mRNA levels of OT and OTR. I The expression of OT in enteric neurons treated with or without conditioned medium were detected by ELISA. The values represent the mean ± SEM of 6 samples and were compared by t-test or one-way ANOVA with Dunnett’s test for multiple comparisons. *p < 0.05, ***p < 0.001 vs. control group