Fig. 3
From: Hypothermia modulates myeloid cell polarization in neonatal hypoxic–ischemic brain injury
Microglia activation peaks 3 days after HI, but is not modulated by HT. Microglia activation was analyzed via immunohistochemistry for Iba-1 on day 1 (a), 3 (b) and 7 (c) after HI, performed in 9-day-old C57BL/6 mice. Animals were exposed to 4 h hypothermia immediately after HI. Controls were kept at physiological body core temperature (normothermia). Iba-1 immunoreactivity was quantified by measurement of positively stained areas in the cortex (Ctx), hippocampus (Hc) and thalamus (Thal). Representative images were acquired in the CA2 region of the hippocampus, scale bar: 50 µm. Dashed lines and grey shadows in the graphs indicate quantiles (i.e., median and 25th, 75th percentiles) of sham-operated animals across all timepoints, statistical comparisons to sham groups were performed for animals of the same timepoint. Detailed time course analyses of sham-operated animals are provided in the Additional file 1: Fig. S1. *p < 0.05, **p < 0.01, #p < 0.05, ## p < 0.01, ### p < 0.001 vs. sham. n = 5 (sham)/timepoint, n = 7 (HI)/treatment and timepoint