Fig. 2

Changes in TRPV4 protein levels in retinas of rats with COH. A Representative immunoblots showing changes in TRPV4 protein levels in control and COH retinal extracts at different postoperative times (G1w, G2w, and G3w). B Bar chart summarizing mean expression levels of TRPV4 at different postoperative times. Data are presented as means ± standard errors of the mean. n = 7, 4, 6, and 5, respectively. **p < 0.01 vs control, ***p < 0.001 vs control. C Immunohistochemistry analysis of changes in TRPV4 protein expression patterns in Müller cells from COH retinas. a1–d1, Immunofluorescence images show TRPV4 protein expression profiles in rat retinal vertical slices collected from control eyes (a1) and profiles in COH retinas collected at different postoperative times (G1w, G2w, and G3w) (b1–d1). a2–d2, Immunofluorescence images show glutamine synthetase (GS) protein (green) and DAPI (blue) staining profiles in the slices depicted in a1–d1. a3–d3 depict merged images and the star symbols in b3–d3 represent obvious merge sites. D Double immunofluorescence staining showing TRPV4 expression when the TRPV4 antibody was pre-adsorbed with its blocking peptide (BP). Scale bar: 50 µm. GCL ganglion cell layer, IPL inner plexiform layer, INL inner nuclear layer, OPL outer plexiform layer, ONL outer nuclear layer. E, Bar graph summarizing mean density of TRPV4 immunofluorescence in retinas under different conditions. n = 4 for all groups. ***p < 0.001