Fig. 5

Müller cells express functional TRPV4. A Immunofluorescence images show that cells with red autofluorescence are Müller cells. A1 Immunofluorescence images show cells with red autofluorescence in rat retinal vertical slices acquired from TdTomato transgenic mouse retina (Tomato). A2 Immunofluorescence images show glutamine synthetase (GS) protein staining (green) in the slices depicted in A1. A3 Immunofluorescence images show DAPI (blue) staining in the slices depicted in A1. A4 shows merged images. Scale bar: 50 µm. B Representative trace recorded from a Müller cell identified by spontaneous red fluorescence, showing that perfusion with GSK101 (10 μM) caused significant depolarization of Müller membrane potential. C Bar chart showing GSK101-induced depolarization of Müller cell membrane potential. n = 4. *p < 0.05 vs control. D Representative trace from cultured Müller cells loaded with Fura-2 and exposed to GSK101 (1 µM, black trace; 10 µM, red trace) or GSK101 in conjunction with HC-06 (blue trace). E Change of the 340/380 ratio in cultured Müller cells treated with either GSK101 alone or GSK101 in the presence of HC-06 (n = 69–125). ***p < 0.001. F Representative cultured Müller cells showing the fluorescence changes in the presence of GSK101 (10 µM). Scale bar: 50 µm