Fig. 4
The expression of synaptic proteins in hippocampus of Cx3cr1CreER IL-10−/−mice. a Western blotting bands of NR2A, NR2B, PSD95, and synaptophysin in littermates and in Cx3cr1CreERIL-10−/−mice. Bar graphs represent densitometric plots of protein expression in littermate and Cx3cr1CreERIL-10−/−mice in NR2A (b), NR2B (c), PSD95 (d), and synaptophysin (e). Each dot represents a mouse. Bars represent mean ± SEM. n = 4 in each group. Significant differences were established by t-test. Spine density of the littermates and the Cx3cr1CreERIL-10−/− mice by Golgi staining (f). The spine density decreased in the pyramidal cells in both apical dendrites (g and i) and basal dendrites (h and j) in hippocampus. Scale (l). Images were captured with a 10 × objective (f) and 100 × objective (g). Bars represent mean ± SEM. n = 6 in each group. Significant differences were established by t-test. *P < 0.05, **P < 0.01, ***P < 0.001