Fig. 7
Upregulation of TRPC5 in hippocampal excitatory neurons improving the learning and memory ability of Cx3cr1CreER IL-10−/−mice. a Schematic of experimental design and schedule. The animal experimental protocol indicated the time course of various interventions utilized during the experiment. b, c Schematics of AAV9-CaMKIIα-TRPC5 (b) and stereotaxic injection in the hippocampus (c). d The expression level of TRPC5 in hippocampus by Western blotting after AAV9 injection (n = 3). There was a significant increase in both escape latency (e) and average distance (f) in KO + TRPC5 compared to the KO group in the training stage. g Representative movement traces from the four groups on the test stage of the MWM task. The mice in the KO + TRPC5 group had more convergent paths in the test stage in the target quadrant, suggesting memory improvements. There was a significant increase in both the platform crossover times (h) and time spent in the target quadrant (i) in the KO + TRPC5 group in the test stage compared with the KO group. Each dot represents a mouse. Bars represent mean ± SEM. n = 5 in each group, Significant differences were established by two-way ANOVA (e, f) and one-way ANOVA (h, i). *P < 0.05, **P < 0.05