Fig. 6

The Toll-like receptor pathway is upregulated in AD patients, and RIPK1 expression is increased in APP/PS1 transgenic mice. A–D Transcriptome sequencing data of the entorhinal cortex, hippocampal cortex, temporal cortex and frontal cortex tissues from AD patients were collected from the GEO database, and GSEA was performed after normalization. E–H The differences in RIPK1 expression in the transcriptome of the entorhinal cortex, hippocampus, frontal cortex and temporal cortex were normalized in AD patients. I–K Nine-month-old APP/PS1 Tg mice were anesthetized and euthanized to obtain the cerebral cortex and hippocampus. I mRNA expression of RIPK1 in the cerebral cortex and hippocampus was detected by qPCR using GAPDH as the internal control. J Protein levels of RIPK1 in the cerebral cortex and hippocampus were elucidated by western blotting with β-actin as the internal control. k ImageJ software was used to semiquantitatively analyze the fold change in RIPK1 relative to β-actin. The data present means ± S.M. of independent experiment. The data present mean ± S.M. of independent experiment. APP/PS1 Tg mice compared with WT mice, ***P < 0.001