Fig. 7

Peripheral pyroptosis affects the neuropathology of 5 × FAD mice. a, b Concentration of IL-1β protein in serum and brain specimens as determined by ELISA in the indicated mice (n = 5). c Expression and activation of GSDMD in spleen specimens as determined by Western blot analysis in the indicated mice. d, e Quantification of relative protein levels shown in c (n = 3). f Immunofluorescence analysis of microglia (IBA1) in hippocampus tissues from indicated mice. g Quantification of relative IOD values shown in f (n = 3). h Immunofluorescence analysis of Aβ plaques (thioflavin S) and neurons (NeuN) in hippocampus tissues from indicated mice. i, j Quantification of relative IOD values shown in h (n = 3). *P < 0.05, **P < 0.01, ****P < 0.001. Data are expressed as means ± SEM. Unpaired t test for a, b, d, e, g, and i, j