Table 2 Antibodies used in Western blotting and immunofluorescence analysis
Antibody | Manufacturer | Catalog number | Host | Dilution |
|---|---|---|---|---|
For Western blotting analysis | Â | Â | ||
SIRT1 | Abcam, UK | ab110304 | Mouse | 1:3000 |
CGRP | Abcam, UK | ab189786 | Rabbit | 1:3000 |
c-Fos | Abcam, UK | ab134122 | Rabbit | 1:1000 |
p-ERK | CST, USA | 4370Â T | Rabbit | 1:1000 |
ERK | CST, USA | 4695Â T | Rabbit | 1:2000 |
p-CREB-S133 | Abcam, UK | ab32096 | Rabbit | 1:3000 |
CREB | CST, USA | 9197Â T | Rabbit | 1:1000 |
Iba1 | Abclonal, China | A1527 | Rabbit | 1:1000 |
iNOS | Proteintech, USA | 18985-1-AP | Rabbit | 1:1000 |
GAPDH | ZEN-BIOSCIENCE, China | 200306-7E4 | Mouse | 1:5000 |
β-actin | ZSGB-Bio, China | TA-09 | Mouse | 1:3000 |
Anti-rabbit IgG (HRP) | ZEN-BIOSCIENCE, China | 511203 | Goat | 1:5000 |
Anti-mouse IgG (HRP) | ZEN-BIOSCIENCE, China | 511103 | Goat | 1:5000 |
For immunofluorescence staining | Â | Â | ||
SIRT1 | Abcam, UK | ab110304 | Mouse | 1:50 |
Iba1 | Wako, Japan | 019-19741 | Rabbit | 1:400 |
NeuN | CST, USA | 24307Â T | Rabbit | 1:400 |
GFAP | Wanleibio, China | WL0836 | Rabbit | 1:50 |
CGRP | Santa Cruz, USA | sc-57053 | Mouse | 1:100 |
c-Fos | Novus Biologicals, USA | NBP2-50057SS | Rabbit | 1:5000 |
Alexa Fluor 488 goat anti-mouse IgG | Beyotime, China | A0428 | Goat | 1:500 |
Cy3-conjugated goat anti-rabbit IgG | Beyotime, China | A0516 | Goat | 1:500 |