Fig. 4

Immunostaining for ATPB (green) and GFAP (red) in the cerebral cortex of 15-week-old SAMR1 mice (A–C) and 5-week-old (D–F), 15-week-old (G–I) and 25-week-old (J–L) SAMP10 mice. (M) Quantification of the immunofluorescent signals. Note that both ATPB and GFAP immuno-reactivity were much higher in the 15-week-old SAMP10 mice than in the two control groups. Although GFAP immuno-reactivity stays higher in the 25-week-old SAMP10 mice, ATPB signal was dramatically reduced to the basal level. The localization of ATPB was observed in GFAP+ astrocytes. Arrowheads indicate ATPB signals. Scale bar: 10 μm. Data are means ± SEs. n = 4 for each group, except for 25-week-old SAMP10 mice (n = 2)