Fig. 2
From: A new method for obtaining bankable and expandable adult-like microglia in mice
Functional validation of NEL-MG. A CD11b+ cells were isolated from cultured NEL for 21 days using an MACS system (NEL-MG) and NEL-MG showed IBA-1, CX3CR1, and TMEM119 IR, but not Ki67 IR. B A phagocytosis assay using microbeads, synaptosome, and amyloid β was conducted to assess NEL-MG. C Migration performance was assessed using a wound healing assay. D Cytokines and chemokines released in the supernatant of NEL-MG in response to LPS challenge. E Transcription in NEL-MG as a baseline (CON) and after LPS stimulation (LPS) (n = 3). The experiment was conducted independently three times. Scale bar = 50 μm. An unpaired t-test was conducted to compare the two groups. ****p < 0.0001 compared to the control