Fig. 4

Validation of NEL-MG isolated from banked NEL. A NEL were banked for about 10 months and thawed. The thawed NEL were cultured for 1, 7, 14, 21, 70 days (d) and NEL-MG were isolated at each time point for flow cytometry. B Banked NEL at different passages (P1, P2, P3, and P4) were thawed and cultured for 7, 14, 21, 40, and 70 d. NEL-MG were isolated at each time point for qPCR (n = 4 per group) (black circle: mean value of P1, P2, P3, and P4) and the data represent mean ± standard error of the mean (SEM), *p < 0.05, **p < 0.001 compared to the pre-banked group. A post hoc test was conducted using Dunnett’s multiple comparison tests. C Immunofluorescence study of IBA-1, CX3CR1, and TMEM119 IR and a phagocytosis assay were conducted using banked NEL-MG (70 d). Scale bar = 50 μm