Fig. 5
Syk deficiency partially attenuates the widespread upregulation of cytokines, chemokines, and other inflammatory mediators in infiltrated neutrophils in the acutely injured spinal cord. A Magnetic isolation enhanced neutrophil purity (> 99%) for qPCR analysis. B Heatmap of the average normalized CT value for 40 genes in neutrophils from the blood of uninjured Sykf/f, as well as the blood and spinal cords of Sykf/f and Sykf/fMRP8-Cre mice at 1 days post-SCI. Gapdh, Actb, and Hprt1 were used as internal controls. N = 3 for uninjured (2F/1M), 3 for Sykf/f (1F/2M), and 3 for Sykf/fMRP8-Cre (0F/3M). n.d. not detected in at least 2/3 samples. Two-way ANOVA with Benjamini, Krieger, and Yekutieli multiple comparisons correction. *q < 0.05 relative to Sykf/f. SCI spinal cord injury)