Fig. 10

Quantification of CD68⁺ and DiI⁺ cells within the sciatic nerves of WT and Lepr^db/db mice. A closer examination of DiI present within the sciatic nerves (SN) of both WT (A) and Lepr^db/db (B) mice was performed to determine if sciatic nerves showed a lower intensity of DiI in CD68⁺ cells as compared to the surrounding tissues. Three CD68⁺DiI⁺ double positive cells were identified from the left and right sciatic nerves (n = 6) of each WT and Lepr^db/db mouse for comparison with three cells from adjacent tissues [muscle (M) (n = 6) or adipose (AT) (n = 6)] on the same slide (C, D). This comparison revealed a significantly lower mean intensity of DiI signal within the CD68⁺ cells of the sciatic nerve from WT (p = 0.0313) and Lepr^db/db (p = 0.0313) mice as compared to muscle and adipose tissue respectively. CD68⁺NE⁺ double positive cells are identified in the sciatic nerve (white arrows) and peripheral tissue (blue arrows) of each image. Scale bar = 100 µm. Error bar represents the standard deviation from six independent measurements