Fig. 6

Identification CD68⁺ and DiI⁺ cells within the hind paws of WT and Lepr^db/db mice. Hind foot histological sections of WT (A) and Lepr^db/db (B) mice injected with PFC-NE 72 h prior to tissue collection were subsequently stained with CD68 and PGP9.5 antibodies (and DAPI) and reviewed for the distribution of CD68⁺ DiI⁺ double positive cells. DiI from the PFC-NE was found within CD68⁺ cells in dermis, hypodermis, bone marrow, and around the neurovascular bundles. Images were taken at 20 × and a composite image was formed using the tile scan feature of the Nikon confocal microscope. Yellow rectangles represent the area of focus around the neurovascular bundles (white arrows) for Fig. 6. Scale bar = 500 µm, MT metatarsals