Fig. 1

Dynamic changes in expression of GFAP and TSPO in mouse COH retinas. a, b Representative immunoblots obtained at different times after operation/sham-operation showing the changes of TSPO and GFAP expression in COH (a) and sham-operated (b) retinas. c, d Bar charts comparing the average relative densities of immunoreactive bands of TSPO (c) and GFAP (d) expression obtained at corresponding post-operational times in COH and sham-operated retinas. All the data are normalized to their corresponding GAPDH and then to the data before operation (control, Ctr). n = 6 for each group. *P < 0.05, **P < 0.01, and ***P < 0.001 vs. the data from the sham-operated group using paired two-tailed Student’s t test (c, d). e Photographs of immunofluorescence staining showing the morphological changes, migration of Iba1-labeled microglia (i1-8) and TSPO expression (t1-8) in retinal vertical slices taken from sham-operated mice (Ctr), and those obtained at different post-operational times (G4d, G1w, G2w, G3w, G4w, G5w, and G6w). e1-8 are enlarged images of the areas encircled by squares in i1-8, and m1-8 are merged images of i1-8 and t1-8. Scale bar: 10 μm for all images. GCL ganglion cell layer, IPL inner plexiform layer, INL inner nuclear layer, OPL outer plexiform layer, ONL outer nuclear layer