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Fig. 8 | Journal of Neuroinflammation

Fig. 8

From: Diphtheria toxin induced but not CSF1R inhibitor mediated microglia ablation model leads to the loss of CSF/ventricular spaces in vivo that is independent of cytokine upregulation

Fig. 8

Increased IBA1 + cells are present in choroid plexus (CP) in Cx3cr1-iDTR and the iDTR mice after 3 day Dtx treatment. Genetic ablation of microglia in Cx3cr1-iDTR mice leads to ablation of IBA1 + microglia in the brain parenchyma (indicated in A and C by arrowheads). However, in the choroid plexus (CP) it leads to increased IBA1 + cells (indicated by arrows in B and D), suggesting a potential inflammatory response and possible infiltration of peripheral macrophages into CP. iDTR mice subjected to Dtx treatment does not result in microglia ablation (indicated in E by arrowheads) but likewise results in increased IBA1 + cells in CP, similar to Cx3cr1-iDTR mice (scale bar = 100 μm left column, scale bar = 20 μm right column). (n = 3–6 for each group, Mean ± SEM, **p < 0.01 and ***p < 0.001, one-way ANOVA). Quantification result is from one cohort of mice with similar results observed from > 2 other independent experiments

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Journal of Neuroinflammation

ISSN: 1742-2094