Fig. 2
From: Immune response and pathogen invasion at the choroid plexus in the onset of cerebral toxoplasmosis
Toxoplasma gondii infection of endothelial and immune cells in the CP. A Animals were infected i.p. with 1 × 105 T. gondii type II PTG-GFP tachyzoites. The brains were isolated at 7 dpi, and coronal sections were immune-stained with anti-GFP (green), anti-CD31 (magenta) and DAPI (cyan). First row indicate the location of the CP (magenta rectangle) in the lateral ventricle (VL) according to the mouse brain atlas. Second row magnify the CP area above. Yellow arrowheads indicate the detection of parasites co-localized with endothelial cells (CD31). Scale bars = 50 µm B Isolated CPs from animals infected i.p. with 1 × 105 PTG-GFP tachyzoites were immune-stained as whole-tissue mount with anti-CD45 (blue), anti-SAG1 (red), and anti-CD31 (green). Animals were infected at the same time, but tissues were analyzed at 3, 5 and 7 dpi. Yellow arrows indicate CD45/SAG1 co-localization, showing immune cells carrying parasites through the BCSFB. Arrowheads indicate co-localization of CD31/SAG1, and asterisks represent SAG1 signal alone. White square indicates magnified area of co-localization signal and orthogonal views of a z-stack analysis. GFP signal plus SAG1 staining were detected in the same channel and colored in red for better visualization. Scale bars = 50 µm. CTX cortex, Cc corpus callosum, CdP caudoputamen, LS lateral septal, VL lateral ventricle