Fig. 3

Effect of a second MPL injection 10 days after the first MPL injection on CSDS-induced anxiety-like behaviors in mice. A Timeline for the evaluation of the effect of the first and second MPL injection with a 10-day interval on CSDS-induced anxiety-like behaviors in mice. B–D Quantitative analysis showing the influence of a second MPL injection (400 μg/kg, 1 day before stress exposure) 10 days after the first MPL injection on the time spent in open arms (B), the number of entries into open arms (C), and the number of entries into total arms (D) in the EPM test in mice treated with or without CSDS and/or MPL (n = 10, **p < 0.01 vs. vehicle, in model 1 and 2; ^##p < 0.01 vs. vehicle + CSDS, in model 1 and 2; ^&p < 0.05 or ^&&p < 0.01 vs. vehicle, in model 3). E, F Quantitative analysis showing the influence of a second MPL injection (400 μg/kg, 1 day before stress exposure) 10 days after the first MPL injection on the time spent in lit side (E) and the number of total transitions (F) in both lit and dark side in the LDT in mice treated with or without CSDS and/or MPL (n = 10, **p < 0.01 vs. vehicle, in model 1 and 2; ##p < 0.01 vs. vehicle + CSDS in model 1 and 2; &p < 0.05 vs. vehicle, in model 3). G, H Quantitative analysis showing the influence of a second MPL injection (400 μg/kg, 1 day before stress exposure) 10 days after the first MPL injection on the time spent in the center region of the open field (G) and the total distance (H) in the OFT in mice treated with or without CSDS and/or MPL (n = 10, **p < 0.01 vs. vehicle, in model 1 and 2; ^##p < 0.01 vs. vehicle + CSDS, in model 1 and 2; ^&&p < 0.01 vs. vehicle, in model 3). Data are shown as mean ± SEM. Comparisons were made by two-way ANOVA with (B, C, E, G) or without (D, F, H) Bonferroni test