Fig. 5

Competitive advantage of higher CX3CR1 expressed microglia during microglial repopulation. a Different percentages of EYFP⁺ F4/80^low, EYFP⁻ F4/80^low, EYFP⁺ F4/80^hi, and EYFP⁻ F4/80^hi microglia in total microglia at Ctrl, day 1, 21 and 42 after Tam injections. b Representative images of Hoechst, Iba-1 and Ki67 staining in the primary cultured microglial. c Bar graph showing the rate of ki67⁺ cells in Iba-1⁺ cells in Cx3cr1^wt/wt, Cx3cr1^{CreER-Eyfp/wt}, Cx3cr1^{CreER-Eyfp/ CreER-Eyfp} primary microglia, and the rate of ki67⁺ cells in Iba-1⁺ cells in Cx3cr1^wt/wt, Cx3cr1^{CreER-Eyfp/wt}, and Cx3cr1^{CreER-Eyfp/CreER-Eyfp} primary microglia after adding 100 ng/ml CX3CL1 in the medium. n = 3–4, mean ± s.d. *p < 0.05 by two-way ANOVA of factor Cx3cr1, no interactions were between factors of Cx3cr1 and CX3CL1. d Migration assay: the left panel showing would density in Cx3cr1^wt/wt, Cx3cr1^{CreER-Eyfp/wt}, Cx3cr1^{CreER-Eyfp/CreER-Eyfp} primary microglia culture. n = 6, mean ± s.d. *p < 0.05, ***p < 0.001 by one-way repeated ANOVA; the right panel showing would density in Cx3cr1^wt/wt, Cx3cr1^{CreER-Eyfp/wt}, Cx3cr1^{CreER-Eyfp/CreER-Eyfp} primary microglia culture after adding 100 ng/ml CX3CL1. n = 6, mean ± s.d. **p < 0.01, ***p < 0.001 by one-way repeated ANOVA; before and after adding CX3CL1 were compared, ^###p < 0.001 by paired t test. e Graph showing cell density in the whole brain of control group, day 0, day 3 day 7 and day 14 after ceasing PLX3397 chow treatment in Cx3cr1^wt/wt, Cx3cr1^{CreER-Eyfp/wt}, Cx3cr1^{CreER-Eyfp/CreER-Eyfp} mice, n = 3–8, mean ± s.d. ^#p < 0.05 by One-way ANOVA