Fig. 7
From: TRPV4 contributes to ER stress and inflammation: implications for Parkinson’s disease
Knockdown of TRPV4 in the SN attenuated inflammation in PD mice. After infused with 0.5 μl AAV-TRPV4 shRNAi bilaterally into the SN of mice for 21 days and administrated with MPTP at 30 mg/kg/day for 7 days, the SN was rapidly dissected out. The protein levels of procaspase-1, IL-18, COX-2 and 5-LOX in the SN of mice were detected by Western blot. A Knockdown of TRPV4 in the SN restored the MPTP-induced the reduction in procaspase-1 (n = 6). B Knockdown of TRPV4 in the SN decreased the MPTP-induced high level of IL-18 (n = 6). C Knockdown of TRPV4 in the SN decreased the MPTP-induced high level of COX-2 (n = 6). D Knockdown of TRPV4 in the SN decreased the MPTP-induced high level of 5-LOX (n = 6). All data are presented as mean ± SEM. Statistical significance: *P < 0.05, **P < 0.01, ***P < 0.001