Fig. 2

An expansion of IL-17⁺IFN-γ⁺ T cells positive for TLR2 and TLR4 was observed in symptomatic CCM patients. Taking into account the representative dot-plots shown in figure (A), the mean frequency of (B) dual IL-17 and IFN-γ-secreting CD4⁺ and CD8⁺ T cells positive for TLR2 and TLR4, before and after Pam3Csk4 (Pam3C, 1 μg/mL) or LPS (100 ηg/mL) addition, was determined by cytometry after acquisition of 200,000 to 300,000 events in samples obtained from asymptomatic (CCM_Asympt, n = 14) and symptomatic (CCM_Sympt, n = 23) CCM patients. Data are shown as mean ± SD of seven independent experiments with 5 to 6 samples per experiment. Significance was calculated by comparing different cell culture conditions from CCM_Asympt and CCM_Sympt groups, and the p values are shown in the figure (Kruskal-Wallis test and Dunn’s test)