Fig.2

Fluoxetine inhibits the production of A1 reactive astrocytes under three different stimulators. A, B Heatmap of A1-special and A2-special transcripts in primary astrocytes after A1 cocktail (IL-1α plus TNF-α plus C1q) stimulation (A) or activated (LPS)-MCM treatment (B) for 24 h as analyzed by RT-qPCR. C–F Protein levels of C3 in astrocyte after A1 cocktail stimulation (C, D) or LPS-MCM treatment (E, F) for 24 h. G Heatmap of A1-special and A2-special transcripts in astrocyte after IL-6 stimulation for 24 h as analyzed by RT-qPCR. H, I Protein levels of C3 in astrocyte after IL-6 stimulation for 24 h. Quantitative data are mean ± s.e. Data were analyzed using two-way ANOVA, then combined with Tukey’s multiple comparison to assess the differences between groups. All data represent the results of three independent experiments. A, B, G: *P < 0.05, **P < 0.01, and ***P < 0.001 vs Control cells treated with PBS (A, G) or non-activated MCM (B); ^#P < 0.05, ^##P < 0.01, and ^###P < 0.001 vs Control cells treated with A1 (A), activated (LPS) MCM (B) or IL-6 (G) stimulation. D, F, I: *P < 0.05, **P < 0.01, and ***P < 0.001