Fig. 1
From: Inflammatory monocytes and microglia play independent roles in inflammatory ictogenesis
The intensity of the acute inflammatory infiltration response to TMEV is titrated by the concentration of the initial viral inoculum. BILs were isolated from LysM:eGFP reporter mice 24 h after intracranial inoculation with 200,000 PFU (A, F, K), 50,000 PFU (B, G, L), 12,500 PFU (C, H, M), or 3125 PFU (D, I, N) of TMEV or injection of vehicle (E, J, O). A–E Forward (FSC) and side (SSC) scatter profiles indicate the presence of more cells with larger FSC in the higher inoculum groups, though the overall number of cells isolated in the BILs preparations are similar. F–J The number of CD45hi cells infiltrating the brain (above the horizontal line) scales with the initial inoculum, while the relative density of CD45mid cells is similar across conditions. K–O The number of GFPmid inflammatory monocytes (between the horizontal lines) also scales with the initial inoculum. P Quantitation of GFPmid inflammatory monocytes and (Q) GFPhi neutrophils. Each dot represents one animal. Bars show mean ± 95% confidence intervals. Percentages in F–O indicate CD45hi, GFPhi, and GFPmid populations relative to total cells in the analysis