Fig. 6
From: Vagus nerve stimulation modulates hippocampal inflammation caused by continuous stress in rats
Effects of cVNS on the production of active form of caspase 3 and 5-HT1A receptors in the hippocampus of CS animals. A, C Western blot analysis of cleaved active form of caspase 3 (A) and 5-HT1A receptors (C) in the hippocampus. Images in A and C are the representatives from four independent experiments. Quantification of band intensity relative to β-actin is plotted in lower panels. ***p < 0.001 (One-way ANOVA). B, D Immunofluorescence staining of active caspase 3 (red in B) and 5-HT1AR (red in D) merged with NeuN (green) in the granule cell layer (GCL) of dentate gyrus (DG) and CA3 and CA1 pyramidal cell layers