Fig. 5

Effects of EDA on astrocyte loss in the CSDS mice Hip and mPFC. A Effect of EDA on astrocyte dysfunction-induced by CSDS in the Hip. Representative images of astrocyte immunostaining for GFAP. Scale bars, 100 μm (upper panel) and 10 μm (lower panel). B Quantification of GFAP-positive cells per mm² in the Hip. C Total branch length. D Branch number. E Effect of EDA on astrocyte loss-induced by CSDS in the mPFC. Representative images of astrocyte immunostaining for GFAP. Scale bars, 100 μm (upper panel) and 10 μm (lower panel). F Quantification of GFAP-positive cells per mm² in the Hip. G Total branch length. H Branch number. All the data are presented as mean ± SEM (n = 3 mice/group, 15 cells/group). *p < 0.05, ***p < 0.001 versus the CON + Vehicle group. ^#p < 0.05, ^##p < 0.01 versus the CSDS + Vehicle group