Fig. 1

Effects of MT5-MMP deficiency and IL-1β treatment on primary cultures of cortical neural cells. A mRNA levels of Mmp24 analyzed by RT-qPCR. Data values were normalized by Gapdh as housekeeping gene. B MT5-MMP levels detected by immunoblot (top panel) with its corresponding quantification (lower panel) normalized with β-actin. Note that MT5-MMP was not detected in MT5^−/− and TgMT5^−/− cells. C mRNA levels of Mmp14 analyzed by RT-qPCR. Data values were normalized by Gapdh as housekeeping gene. D Representative confocal micrographs of primary neuronal cultures from WT mice treated or not with IL-1β and labeled with astrocytic marker GFAP (green) and neuronal marker β-III tubulin (red). Nuclei are stained with Hoechst (blue). Scale bar: 30 μm. E and F Detection of β-III tubulin and GFAP levels by immunoblots (top panels) with their corresponding quantifications (lower panel) normalized with β-actin. G Histogram showing the quantification of cell viability using the MTT assay. A–C and E–G, Black bars represent control (untreated) conditions and grey bars IL-1β treated conditions (10 ng/mL for 24 h). Values for A–C are the mean ± SEM of 6–8 independent cultures by genotype, for E and F of 4 independent cultures by genotype and for G of 3-7 independent cultures by genotype. Values are presented as % of the control. ^#p < 0.05, ^##p < 0.01 and ^###p < 0.001 between genotypes. ANOVA followed by post hoc Fisher’s LSD test. IB Immunoblot, O.D. optical density, A.U. arbitrary units