Fig. 2

Effects of MT5-MMP deficiency on IL-1β-mediated neuroinflammation in cortical neural cell cultures. A–D Analyses of Il-1β, Ccl2, Il-6 and Tnfa basal mRNA expression in primary neural cells by RT-qPCR and normalized by Gapdh as housekeeping gene. Note the consistent significant decrease of Il-1β, Ccl2 and Il-6 mRNA levels in TgMT5^−/− cells compared to Tg. E–H Analyses of Il-1β, Ccl2, Il-6 and Tnfa mRNA expression in primary neural cells by RT-qPCR and normalized by Gapdh as housekeeping gene. Black bars represent control (untreated) conditions and grey bars IL-1β treated conditions (10 ng/mL for 24 h). Values for A–H are the mean ± SEM of 6–10 independent cultures by genotype. *p < 0.05, and ***p < 0.001 between untreated and treated cultures in the same genotype; ^#p < 0.05 and ^##p < 0.01 between genotypes. ANOVA followed by post hoc Fisher’s LSD test. A.U. arbitrary units