Fig. 8

Activating S100B/RAGE signal promotes ADAM17 translocation in RAECs. A Representative confocal images showing the subcellular localization of ADAM17 and GM130 (the Golgi complex-associated protein) in RAECs treated with stretch injured-conditioned medium, exogenous S100B, and injured medium plus S100B inhibitor ONO-2506 or RAGE inhibitor FPS-ZM-1. Scale bar = 20 μm. The immunofluorescence intensity of GM130⁺/ADAM17⁺ in cell from five fields per sample in each group was quantified and reported as relative fluorescence units (RFUs). *p < 0.05 compared with control group, ^#p < 0.05 compared with conditioned medium-treated group, n = 3. B Representative confocal images showing the subcellular localization of ADAM17 and vWF in RAECs treated with stretch injured-conditioned medium, exogenous S100B, and injured medium plus ONO-2506 or FPS-ZM-1. Scale bar = 20 μm. The immunofluorescence intensity of vWF⁺/ADAM17⁺ in cells from five fields per sample in each group was quantified and reported as relative fluorescence units (RFUs). *p < 0.05 compared with control group, ^#p < 0.05 compared with conditioned medium-treated group, n = 3